Objective: to prepare slides of animal cell and plant cell using the correct staining technique introduction: all living things are made up of basic units called cells hypothesis: inner cheek cells have a nucleus, plasma membrane and cytoplasm where as leaf epidermal cells have a nucleus, plasma. Classroom activity for the nova program cracking the code of life: in see your dna, students extract human dna from cheek cells grades 6-8, 9-12. Cheek cells are eukaryotic cells (cells that contain a nucleus and other organelles within enclosed in a membrane) that are easily shed from the mouth lining. Pcr amplification of cheek cell dna will amplify a piece of dna that is 941 bp long the absence of the alu insert produces a 641 bp dna fragment alu after pcr. In this lab activity students isolate genomic dna from their cheek cells on the inside of their mouths students then remove the dna from those cheek cells it shows the dna is in every cell in the body and can be extracted easily. How to find cheek cells with a microscope, a step by step guide. We will first collect cheek cells by swishing a sports drink in our mouths and using our teeth to scrape cells off our dna extraction from human cheek cells.
They are the basic building blocks of all animals and plants these photographs show cells seen through a microscope cheek cells these are cheek cells. Epithelium lines surfaces and cavities, such as the mouth, throughout the body cheek cells consist of a specific type of epithelium: stratified squamous epithelium. Cheek cell aim to prepare a slide of cheek cell and observe it under the microscope materials required flat-edge toothpicks, microscope, slide, water, paper towels, methylene blue, cover slip procedure place a drop of water on a microscope slide. As in all animal cells, the cells of the human cheek do not possess a cell wall a cell membrane that is semi-permeable surrounds the cytoplasm unlike plant cells, the cytoplasm in an animal cell is denser, granular and occupies a larger space. Cheek cell lab purpose: to find out what an animal cells look like under a microscope hypothesis: if i look at my cheek cell scraping under a microscope and put iodine on it, then i will see the different parts of the cell including the nucleus, cytoplasm, and cell. Extracting dna from cheek cells: a classroom experiment for year 7 upwards dr kathryn scott research administrator, zitzmann group, department of.
To get the dna out of your cheek cells you need to break open both the cell membranes and the nuclear membranes cell membranes and nuclear membranes consist primarily of lipids dishwashing detergent, like all soaps, breaks up lipids this is why you use detergents to remove fats (which are lipids) from dirty dishes. Isolation of cheek cell dna 1 pour 10 ml of saline solution (09% nacl) into a 50 ml blue centrifuge tube 2 pour the saline into your mouth and vigorously rinse your mouth for. How to make a cheek cell slide: 1 gently scrape the inside lining of your cheek with a clean toothpick 2 smear toothpick onto clean glass slide. For the cheek cells with methylene blue, do the same steps as the cheek cells with water but instead of adding three drops of water, add three drops of methylene blue a) for the onion cell, use the tweezers to get a little piece of an onion layer and put it onto the slide.
Get an answer for 'what type of cells are cheek cells' and find homework help for other science questions at enotes. Cheek cells are epithelial cells that line the interior surface of our mouths all epithelial cells share the same characteristics the base layer of cells in an epithelial structure are not actually cells, but a sticky layer on which the cells anchor the other surface of the epithelial cell.
The nucleus of the inside cheek cells serves the same purpose as the nucleus of every cell, storing the dna that helps the rest of the cell perform its function the. In cheek cells, examine some of your own cheek cells under a microscope explore what your own cells look like up close cheek cells is part of the cell lab the cell lab is.
Extracting dna from cheek cell extracting dna from human cheek cells: conclusion the hypothesis of my experiment, if the strawberry dna product was strandlike yet. Start studying all about cells learn vocabulary, terms, and more with flashcards, games, and other study tools why was stain added to the cheek cells. Methylene blue can be used in biology as a stain to indicate whether cells are dead or alive the stain cannot penetrate live cells however, in dead cells, it can penetrate the cell membrane and the cells will be stained blue it is also used to study rna and dna in gel or under the microscope. Cuboidal epithelium has cells whose height and width are approximately the same human cheek cells (nonkeratinized stratified squamous epithelium) 500x. Crushing physically crushing cheek cells will release the dna that is inside of them cells are made of membranes and an internal protein skeleton. The cheek cells contain dna but to get enough dna to view with your kit, you need to collect lots of cheek cells the more you swish and scrape, the more cells you will. To prepare slides of cheek cells and onion cells.